The genetic editing tool, CRISPR-Cas9, is making greater strides regarding RNA linked diseases. The knowledge of how CRISPR-Cas9 can affect DNA has increased over the past couple of years. By targeting the DNA with CRISPR-Cas9 scientists have found new ways to modify protein production and treat certain diseases, which led to editing genes. However, now there is inquiry about what would occur if CRISPR-Cas9 targeted RNA. Many diseases are linked to RNA and by targeting RNA with CRISPR-Cas9 we could find new treatments to fight off cancer, autism, and X-syndrome. Researchers at University of California, San Diego School of Medicine have been able to accomplish targeting the RNA. Gene Yeo, PhD, associate professor of cellular and molecular medicine hopes to use this technique to fix RNA behavioral diseases.
RNA can affect when and where proteins will be produced, but if the RNA transport is deficient than it can cause diseases from autism to cancer. Evaluating RNA movement will allow new treatments to be found. Yeo and colleagues at the University of California, Berkeley, have created RCas9, which is targeting RNA in live cells. They were able to do so by altering certain features of the CRISPR-Cas9. A short nucleic acid, PAMmer, that they designed was used to direct CRISPR-Cas9 to an RNA molecule. They then targeted RNA that encodes certain proteins which were ACTB, TFRC, and CCNA2. The CRISPR-Cas9 would combine with a fluorescent protein to reveal the movement of RNA into stress granules. This allowed the team to track RNA through the live cells without using artificial tags, which are normally used to track RNA.
CRISPR-Cas9 is opening new ways to find out more information to fix diseases regarding DNA and now RNA. There has been controversy regarding CRISPR-Cas9 because it is a tool to edit genetic material, but in this case it is helping us fight off diseases that have been affecting lives for ages. Do you believe that CRISPR-Cas9 should only be used for certain cases or that people should be able to use it freely?